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7 The emergence of very efficient antiviral regimens for the treatment of chronic hepatitis B and C infections has brought to the forefront the need for tackling the most common and most commonly ignored NAFLD-related liver disease

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Protein concentrations were determined by measuring the absorbance at 280 nm, A 280 , with calculated extinction coefficients as provided by the ProtParam server ( according to the formula ε 280 = 1490 × n Tyr + 5500 × n Trp + 125 × n Cystine M −1 cm −1 , plus a further increment of 7600 M −1 cm −1 to account for the contribution of the Pap side chain in this spectral region, if applicable