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After a 20-min incubation with immunol staining blocking buffer (Beyotime, Shanghai, China), cells were continuously incubated with mouse anti-SMA (1:100, Abcam) and rabbit anti-CD133 (1:100, Abnova) 44 at 4 °C overnight
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Comparative studies have found GHK-Cu to be as effective as retinol and vitamin c in improving visible signs of aging, but with significantly lower rates of skin irritation
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