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Fig.2 GST tag resin GST resin technical parameters Buffer recommendation Equilibration buffer: 10-20mM PB/50-100mM Tris-HCl + 0.14-0.4M NaCl，pH6.2-8.0 Elution buffer：50mM Tris-HCl+10-60mM reduced glutathione, pH8.0-9.0 Tips: To improve purity of target molecules, add reducing agent in binding buffer and elution buffer, i.e
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Like the rest of this blend, the fat-loss evidence is preclinical, and human outcome data does not yet exist
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