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SC maximum per site: 1–2 mL (optimal under 1 mL) IM deltoid maximum: 2 mL IM ventrogluteal maximum: 5 mL IM vastus lateralis maximum: 3 mL Most peptide doses reconstitute to 0.1–0.5 mL — well within SC range Split large volumes across multiple sites if exceeding limits Practical Laboratory Protocol Always follow institutional biosafety protocols and use appropriate personal protective equipment when handling injectable preparations