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Binding kinetics of FITC-z13 to CNGB A synthetic MYC epitope peptide (Anaspec) was biotinylated using sulpho-NHS-biotin (Pierce) and purified using a reverse-phase high-performance liquid chromatography column eluted with a linear gradient from 30 to 70% acetonitrile in water containing 0.1% trifluoroacetate over 20 min using Shimadzu SCL-10A HPLC