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These experiments indicated that, in addition to requiring Cys-dependent de novo biosynthesis of GSH for survival (see above), TrxR1/Gsr-null livers require de novo biosynthesis of Cys via transsulfuration, and that they cannot effectively utilize cystine as a hepatic Cys source [107]
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Clinicians prescribing or supervising either agent should document informed consent that explicitly covers the investigational nature of the therapy, the absence of FDA approval, and the specific risks outlined above
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