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Cat# C0042, Beyotime, China) assay is based on WST-8 and was used to assess cell proliferation ( Scratch Wound Healing Assay The monolayers of HDFs were incubated at 37°C for 2 h with mitomycin-C (5 μg/ml, Cat# S8146, Selleck, United States) to inhibit cell proliferation before wounded with a 1-ml plastic pipette tip and then washed with phosphate-buffered saline (PBS) to remove redundant cell debris

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If you are not comfortable with precise syringe technique, the 3.0 mL or 4.0 mL reconstitution gives you more room for error