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Assay of caspase-3 activity GSH depleted HepG2 cells with or without NAC (2×10 6 cells/well) were treated with different concentrations of ASA as described above and the caspase-3 substrate, DEVD-pNA was used to assay the protease activity colorimetrically as described in the vendor's protocol (R & D Systems)

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Thomas A, Görgens C, Guddat S, Thieme D, Dellanna F, Schänzer W, Thevis M