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The protein yield of isolated S219V mutant TEV protease with the background mutations was 10 mg·L −1 of culture media
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Similar to glucose, 2DG is transported into cells and is phosphorylated to 2DG-6-phosphate at the 6′ position by hexokinase (HK), but this phosphorylated substrate cannot be converted to fructose-6-phosphate by phosphoglucose isomerase (PGI), and is thus trapped in the cell
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