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Medium composition was based on Rennie medium 42 , with some modifications, including 800 mg L −1 K 2 HPO 4 , 200 mg L −1 KH 2 PO 4 , 100 mg L −1 NaCl, 98 mg L −1 MgSO 4 , 60 mg L −1 CaCl 2 ·2H 2 O, 1 mg L −1 NaFe-EDTA, 1 mg L −1 Na 2 MoO 4 ·2H 2 O, 10 mg L −1 p -aminobenzoic acid, 5 mg L −1 biotin, 100 mg L −1 Bacto Yeast Extract, and appropriate carbon sources, specifically 7.5 g L −1 glucose in KD medium

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Finally, we review studies on TCM modulation of BA metabolism and ferroptosis to improve lipid peroxidation and metabolic disorders, providing timely insights into innovative therapeutic strategies for NAFLD