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In order to detect a medium effect size of 0.625 (average accuracy increase of 0.08 and standard deviation of 0.128) for the primary outcome (MIFT accuracy), with an intra subject correlation of 0.3, a power of 80% a false positive rate set to 5%, and assuming a 30% dropout rate, the target sample size was 44 participants

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Size:50 ml.

Methods Animals Thirty female Sprague–Dawley rats weighing 200 ± 10 g were purchased from the Chinese Academy of Medical Sciences (Shanghai, China)