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The goal of reconstitution is to gently reintroduce a solvent—in this case, bacteriostatic water—without damaging the peptide's integrity

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Furthermore, using contrast variation, it was shown that the nucleosome unfolding induced by a decrease in ionic strength represents a complex process, where DNA became partially unfolded below the transition point, whereas only small conformational changes occur in the protein core (Uberbacher et al., 1983)

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