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FerroOrange quantitative analysis Labile intracellular Fe²⁺ was measured using the fluorescent probe FerroOrange (Sigma-Aldrich, #SC210) and flow cytometry

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Metabolomics, the comprehensive study of small molecules (metabolites) within biological systems, is particularly well-suited for this research due to the fact that metabolomics effectively reveals how ammonia nitrogen stress disrupts liver metabolism by detecting changes in metabolites and affected pathways, such as energy metabolism and antioxidant defense