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In double-blind, placebo-controlled trials, daily application of GHK-Cu cream at 3% concentration for 12 weeks produced a 70% increase in skin collagen thickness as measured by ultrasound imaging, a 40% increase in skin elasticity, and visible reduction in fine lines and wrinkles

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In parallel with phosphorylation of MAPK, the effect of GHK-Cu on phosphorylation of NF-κB p65 were measured by Western blotting