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Experiments Human aortic endothelial cells at passage 7 were plated in 1% (w/v) gelatin-precoated 96-well plates or 10-cm dishes with Medium 199 (Sigma-Aldrich) supplemented with 20% (v/v) FBS (Sigma-Aldrich), 2 mM glutamine, 50 U/ml penicillin, 50 μg/ml streptomycin, and 1 ng/ml human basic fibroblast growth factor (Roche)