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(b) Similarly, a transfer of radioactivity to P450 could be demonstrated from labeled hemoglobin heme in vivo (Wyman et al., 1986) or when a P450 enzyme containing prelabeled heme was mixed with another unlabeled P450 enzyme in vitro and the radioactivity was then determined in both after resolving them from each other (Sadano and Omura, 1983
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