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BH4 availability is largely driven by the Mg 2+ , Zn 2+ , and NADPH dependent de novo synthesis pathway composed of the rate limiting enzyme GTP cyclohydrolase I (GCH1) together with 6-pyruvoyl-tetrahydropterin synthase and sepiapterin reductase

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The larger the b ∗ value is, the yellower the color ( Enzyme Activity Assay Mycelium and fruiting bodies were ground to a fine powder in liquid nitrogen, fully mixed in extraction buffer, and centrifuged at 4°C at 15,000 × g for 20 min