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In mouse primary submandibular gland (SMG) cells, mitochondrial ROS levels were increased by days 1–3 post-IR with a reduction in ROS levels observed in cells deficient in transient receptor potential melastatin-related 2 (TRPM2), a calcium-permeable cation channel that is activated by oxidative stress and the DNA damage responsive protein, poly (ADP-ribose) polymerase 1 (PARP1), which correlates with improved salivary secretory function post-IR [45]

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They take a BAC measurement from a blood or breath test and work backward, using the average rate of alcohol elimination to calculate what the BAC might have been hours earlier