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ab208942, Abcam) in PBS containing 0.3% (vol/vol) Triton X-100, 0.25% (wt/vol) λ-carrageenan, and 5% (vol/vol) donkey serum (PBS-XCD)
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The drop in leptin levels during fasting contributes to the increased motivation, while leptin administration reduces the rewarding value of food and thus the motivation to eat [70,71]
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