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ROA is also used in the characterization of secondary structure of proteins and polymers, as illustrated by early studies on α-helical and unordered polylysine (Wilson et al., 1996), polyglutamic acid in α-helical and disordered conformations, bovine α-lactalbumin in native and acid molten globule states, and human immunoglobulin (Hecht et al., 1999)