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What Needle Size for BPC 157? Our Team's Expert Breakdown
After the total protein concentrations were detected using a bicinchoninic acid (BCA) protein assay (Beyotime, China), an equal amount of protein from each sample was resolved by SDS-PAGE on 10% SDS-polyacrylamide gels and transferred to a polyvinylidene difluoride (PVDF) membrane for immunoblot analyses
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Immunofluorescence After the behavioral assessments, the rats were euthanized by cervical dislocation and decapitation
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