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Luminescence was quantified using a SpectraMax iD5 multi-mode microplate reader with SoftMax Pro software (version 7.1, Molecular Devices, San Jose, CA, Data were normalized to vehicle and forskolin only control values and analyzed using nonlinear regression with Prism 8.4 (Graphpad, San Diego, CA)

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TB-500 Mechanisms • Binds to actin, a protein crucial for cell movement and regeneration