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Unit Of Measure:10G
Individual clones containing LacAnc100 and LaccID were inoculated into 50 μl of SC minimal medium containing 6.7 g l −1 yeast nitrogen base (BD, 291940), 1.92 g l −1 yeast synthetic dropout medium supplement without uracil (Merck, Y1501), 20 g l −1 d-raffinose (Merck, R0250) and 25 μg ml −1 chloramphenicol (Merck, C0378) in a sterile 96-well plate (Enzyscreen, CR1496c)
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