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Viability was assessed by Zombie UV Fixable Viability Dye (BioLegend) and cytokine production was measured by intracellular cytokine staining after 3 h stimulation with PMA (50 ng ml − 1 ) (BioGems) and ionomycin (1 µg ml − 1 ) (BioGems) in the presence of brefeldin A (1 μg ml − 1 ) (GolgiPlug, BD Biosciences)