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PAC, an evolutionarily conserved membrane protein, is a proton-activated chloride channel

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Then the slides were stained with propidium iodide, as previously described ( Measurement of Cleaved Caspase 3 in Heart Homogenates Heart homogenates for the measurement of cleaved caspase 3 in mice exposed to either air or WPS with or without GA treatment were prepared as described before ( Statistics One-way analysis of variance (ANOVA) and all graphs were produced using GraphPad Prism Version 5 for Windows software (GraphPad Software Inc., San Diego, CA, United States)