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Number of Vials:4 Vials (Differentiated Results)
(17)制备粗品 (17) Preparation of crude product 把吹干的Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin倒入玻璃的切割瓶中,加入预冷的切肽试剂(TFA9.5ml,H 2 O0.5ml),常温反应1.5小时,过滤除去树脂,加冰乙醚500ml沉淀,3500转3分钟离心收集沉淀物,用乙醚洗涤3次,用纯水溶解后,低温冷冻干燥24小时,获 得BPC157肽粗品1.23g,经HPLC测定纯度为78%。 Pour the dried Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin into the glass Add pre-cooled peptide cutting reagent (TFA9.5ml, H 2 O0.5ml) to the cutting bottle, react at room temperature for 1.5 hours, filter to remove the resin, add 500ml of glacial ether to precipitate, centrifuge at 3500 for 3 minutes to collect the precipitate, and diethyl ether After washing 3 times, dissolving with pure water, and lyophilizing at low temperature for 24 hours, 1.23 g of crude product of BPC157 peptide was obtained, and the purity was 78% as determined by HPLC
This health supplement acts as an important part of the natural antioxidant system and may be able to help with supporting normal cell growth to help promote optimal overall health and wellbeing