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UV (UV) detection was set to 361 nm, the maximum absorption wavelength of vitamin B 12 forms (cyanocobalamin at 361 nm and hydroxocobalamin at 355 nm), 11 , 12 and where there is minimal absorption by protein
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The cells were subcultured two–three times per week to obtain 10 to 15 passages, which were utilised in experiments to establish a co-culture system with PC12 cells (PNS in vitro model) [36,37]
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