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Herein, we report the first case of a 23-year-old woman with vitiligo after receiving a PCL-based collagen stimulator filler
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Reactions were stopped at various timepoints by quenching with gel loading buffer (NuPAGE LDS sample buffer, Life Technologies), loaded onto 4–12% SDS-PAGE gels and transferred onto PVDF membranes for western blotting using site-specific primary antibodies, H3K9ac, H3K27ac, H3K14ac and H4 (Cell Signalling #9649, #D5E4, Sigma-Aldrich #07-353, and Abcam #ab7311 respectively)