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Approximately 30 mg of tissue was homogenized in 1 mL of TRIzol reagent in the presence of 0.1 M 8-mercaptoethanol (Life Technologies, Gaithersburg, MD, USA) as a reducing agent
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To further validate, we measured the concentration of nicotine, cotinine and 3′-hydroxy-cotinine (OH-cotinine) in the exposed wells using mass spectrometry
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