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Non-enzymatic S-glutathionylation reactions depend upon the GSH/GSSG ratio within the cell and occur through thiol-disulfide exchange reactions between GSSG and the protein cysteinyl residues, or by the reaction of GSH with an oxidized thiol derivative such as S-nitrosyl (-SNO), thiyl radical (-S•), or sulfenic acid (-SOH) (Figure 3) [56]
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